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[1]何慧怡,樊丽娜,齐永文,等.灭草烟在甘蔗组培苗生根阶段浓度筛选试验[J].甘蔗糖业,2019,(02):013-17.
 HE Hui-yi,FAN Li-na,QI Yong-wen,et al.Imazapyr Concentration Screening at Rooting Stage of Sugarcane Tissue Culture[J].Sugarcane & Canesugar,2019,(02):013-17.
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灭草烟在甘蔗组培苗生根阶段浓度筛选试验
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《甘蔗糖业》[ISSN:1005-9695/CN:44-1210/TS]

卷:
期数:
2019年02期
页码:
013-17
栏目:
研究与开发
出版日期:
2019-04-30

文章信息/Info

Title:
Imazapyr Concentration Screening at Rooting Stage of Sugarcane Tissue Culture
文章编号:
1005-9695(2019)02-0013-05
作者:
何慧怡樊丽娜齐永文*劳方业陈勇生
(广东省生物工程研究所(广州甘蔗糖业研究所) 广东省甘蔗改良与生物炼制重点实验室,广东广州510316)
Author(s):
HE Hui-yi FAN Li-na QI Yong-wen LAO Fang-ye CHEN Yong-sheng
(Guangdong Provincial Bioengineering Institute (Guangzhou Sugarcane Industry Research Institute)/Guangdong Key Lab of Sugarcane Improvement & Biorefinery, Guangzhou 510316)
关键词:
灭草烟甘蔗筛选浓度
Keywords:
Imazapyr Sugarcane Concentration screening
分类号:
S566.1
文献标志码:
A
摘要:
以ROC22增殖阶段的组培苗为材料,探究了不同浓度灭草烟处理对小苗生根及生长的影响。灭草烟处理浓度为0.5 μmol/L时,生根率低于50%,小苗没有假茎,绿叶数量显着减少。处理浓度为1.2 μmol/L以上时,生根率为0。当处理浓度为1.6~2.5 μmol/L时,接近半致死浓度。综合致死率和苗的生根及生长情况分析,灭草烟浓度为1.2 μmol/L时,ROC22组培苗能部分存活但不能长根。因此,适合ROC22组培苗生根阶段的灭草烟浓度为1.2 μmol/L。
Abstract:
With the callus growth of sugarcane variety ROC22 as materials, this paper determined the concentration of imazapyr in rooting stage of ROC22. The results showed that the rooting plantlet percentage of the sugarcane was less than 50% when the imazapyr concentration was 0.5 μmol/L, and the plantlet had no pseudo-stem and much less green leaves. The rooting plantlet percentage was zero when the imazapyr concentration was 1.2 μmol/L. As the imazapyr concentration was 1.6~2.5 μmol/L, it was close to the semi-lethal concentration. Based on some factors such as fatality rate and rooting ratio as well as plantlet growth, the suitable imazapyr concentration at the rooting stage for ROC22 was 1.2 μmol/L.

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备注/Memo

备注/Memo:
收稿日期:2019-03-13;修回日期:2019-04-18
基金项目:国家糖料产业技术体系(CARS-170107);广东省科技计划(2016A030303018、2014B070705002、2016A030313415);广东省科学院实施创新驱动发展能力建设专项资金项目(2017GDASCX-0105)
作者简介:何慧怡(1981-),女,高级农艺师,主要研究方向为甘蔗遗传育种;E-mail:emil06@126.com
*通讯作者:齐永文(1978-),男,博士,副研究员;E-mail:yongwen2001@163.com
引文格式:何慧怡,樊丽娜,齐永文,等. 灭草烟在甘蔗组培苗生根阶段浓度筛选试验[J]. 甘蔗糖业,2019(2):13-17.
更新日期/Last Update: 2019-04-29