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 HUANG Qiong,WANG Qing,WU Qi-hua,et al.Effects of Nitrification Inhibitors on Nitrification and Ammonia Oxidizers Abundance and Community Structure in an Acidic Red Soil[J].Sugarcane & Canesugar,2019,(02):018-26.





Effects of Nitrification Inhibitors on Nitrification and Ammonia Oxidizers Abundance and Community Structure in an Acidic Red Soil
黄 琼12王 庆1*吴启华1李 爽1黄 莹1陈迪文1江 永1
(1广东省生物工程研究所(广州甘蔗糖业研究所) 广东省甘蔗遗传改良与生物炼制重点实验室,广东广州510316;2国家糖业质量监督检测中心,广东广州510316)
HUANG Qiong12 WANG Qing1 WU Qi-hua1 LI Shuang1 HUANG Ying1 CHEN Di-wen1 JIANG Yong1
(1Guangdong Provincial Bioengineering Institute (Guangzhou Sugarcane Industry Research Institute), Guangdong Key Laboratory of Sugarcane Improvement and Biorefinery, Guangzhou 510316; 2China Sugar Inspection Center, Guangzhou 510316)
Acidic red soil Nitrification process AOB AOA Nitrification inhibitor
以一种典型酸性红壤为研究对象,在微宇宙培养条件下,研究硝化抑制剂双氰胺(DCD)和乙炔(C2H2)对酸性土壤硝化过程以及氨氧化微生物丰度和群落结构的影响。结果表明:硫酸铵(铵态氮肥)施加刺激了土壤硝化过程,硝化抑制剂处理显着抑制了硝化作用并且C2H2对硝化过程的抑制作用比DCD更强。研究还发现氨氧化细菌(Ammonia-oxidizing Bacteria,AOB)的丰度与NO3--N的浓度呈显着正相关关系,而氨氧化古菌(Ammonia-Oxidizing Archaea,AOA)的丰度与NO3--N的浓度呈显着负相关关系。末端限制性片段多态性(T-RFLP)分析和主坐标分析(PCoA)表明单施硫酸铵处理改变了AOB和AOA的群落结构。硝化抑制剂处理对AOA和AOB的群落结构没有显着影响。这些结果表明高氨氮条件下AOB是土壤硝化过程的主要执行者,硝化抑制剂通过抑制AOB的丰度从而抑制硝化过程。
Effects of 2 different nitrification inhibitors (NIs, DCD and C2H2) on nitrification process, ammonia oxidizers abundance as well as their community structure in an acidic red soil were studied by cultivation experiment. Our results showed that ammonium-fertilizer application significantly increased the net nitrification rates, but were significantly inhibited by both NIs, and the inhibitory effect of C2H2 was significantly greater than that of DCD. AOB amoA gene abundance was positively correlated with NO3- concentration, while AOA amoA gene abundance was negatively correlated with it. Application of ammonium-fertilizer significantly changed the diversity of the AOB community in the sugarcane soil. AOA community structure was shifted in the ammonium treatment compared to the control. The application of NIs did not change the diversity of ammonia oxidizers. These results indicated that the application of ammonium-fertilizer combined with nitrification inhibitor mainly regulated AOB amoA gene abundance in high ammonium soil.


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引文格式:黄琼,王庆,吴启华,等. 硝化抑制剂对酸性红壤硝化作用及氨氧化微生物丰度和群落结构的影响[J]. 甘蔗糖业,2019(2):18-26.
更新日期/Last Update: 2019-04-29